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Differential Responses of Eubacterial, Mycobacterium , and Sphingomonas Communities in Polycyclic Aromatic Hydrocarbon (PAH)‐Contaminated Soil to Artificially Induced Changes in PAH Profile
Author(s) -
Uyttebroek Maarten,
Spoden Astrid,
OrtegaCalvo JoseJulio,
Wouters Katinka,
Wattiau Pierre,
Bastiaens Leen,
Springael Dirk
Publication year - 2007
Publication title -
journal of environmental quality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 171
eISSN - 1537-2537
pISSN - 0047-2425
DOI - 10.2134/jeq2006.0471
Subject(s) - phenanthrene , pyrene , sphingomonas , environmental chemistry , sphingomonas paucimobilis , bioavailability , polycyclic aromatic hydrocarbon , chemistry , soil contamination , microbial population biology , arthrobacter , soil water , bacteria , biology , ecology , organic chemistry , biochemistry , 16s ribosomal rna , bioinformatics , genetics , gene , enzyme
Recent reports suggest that Mycobacterium is better adapted to soils containing poorly bioavailable polycyclic aromatic hydrocarbons (PAHs) compared to Sphingomonas To study this hypothesis, artificial conditions regarding PAH profile and PAH bioavailability were induced in two PAH‐contaminated soils and the response of the eubacterial, Mycobacterium , and Sphingomonas communities to these changed conditions was monitored during laboratory incubation. Soil K3663 with a relatively high proportion of high molecular weight PAHs was amended with phenanthrene or pyrene to artificially change the soil into a soil with a relatively increased bioavailable PAH contamination. Soil AndE with a relatively high proportion of bioavailable low molecular weight PAHs was treated by a single‐step Tenax extraction to remove the largest part of the easily bioavailable PAH contamination. In soil K3663, the added phenanthrene or pyrene compounds were rapidly degraded, concomitant with a significant increase in the number of phenanthrene and pyrene degraders, and minor and no changes in the Mycobacterium community and Sphingomonas community, respectively. However, a transient change in the eubacterial community related to the proliferation of several γ‐proteobacteria was noted in the phenanthrene‐amended soil. In the extracted AndE soil, the Sphingomonas community initially developed into a more diverse community but finally decreased in size below the detection limit. Mycobacterium in that soil never increased to a detectable size, while the eubacterial community became dominated by a γ‐proteobacterial population. The results suggest that the relative bioavailability of PAH contamination in soil affects bacterial community structure but that the behavior of Mycobacterium and Sphingomonas in soil is more complex than prospected from studies on their ecology and physiology.