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Determination of Polyacrylamide in Soil Waters by Size Exclusion Chromatography
Author(s) -
Lu Jianhang,
Wu Laosheng,
Gan Jianying
Publication year - 2003
Publication title -
journal of environmental quality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 171
eISSN - 1537-2537
pISSN - 0047-2425
DOI - 10.2134/jeq2003.1922
Subject(s) - polyacrylamide , chemistry , size exclusion chromatography , dissolved organic carbon , absorbance , detection limit , chromatography , aqueous solution , environmental chemistry , elution , leachate , soil water , gel permeation chromatography , polymer , environmental science , soil science , biochemistry , polymer chemistry , enzyme , organic chemistry
Determination of polyacrylamide (PAM) concentration in soil waters is important in improving the efficiency of PAM application and understanding the environmental fate of applied PAM. In this study, concentrations of anionic PAM with high molecular weight in soil waters containing salts and dissolved organic matter (DOM) were determined quantitatively by size exclusion chromatography (SEC) with ultraviolet (UV) absorbance detection. Polyacrylamide was separated from interferential salts and DOM on a polymeric gel column eluted with an aqueous solution of 0.05 M KH 2 PO 4 and then detected at a short UV wavelength of 195 nm. Analysis of PAM concentrations in soil sorption supernatants, soil leachates, and water samples from irrigation furrow streams showed that SEC is an effective approach for quantifying low concentrations (0–10 mg L −1 ) of PAM in waters containing soil DOM and salts. The method has a lower detection limit of 0.02 μg and a linear response range of 0.2 to 80 mg L −1 Precision studies gave coefficients of variation of <1.96% ( n = 4) for >10 mg L −1 PAM and <12% ( n = 3) for 0.2 to 3 mg L −1 PAM.