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Using Minced Horseradish Roots for the Treatment of Polluted Waters
Author(s) -
Roper J. Chadwick,
Dec Jerzy,
Bollag JeanMarc
Publication year - 1996
Publication title -
journal of environmental quality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 171
eISSN - 1537-2537
pISSN - 0047-2425
DOI - 10.2134/jeq1996.00472425002500060011x
Subject(s) - horseradish peroxidase , chemistry , phenols , sorption , aqueous solution , chromatography , precipitation , human decontamination , adsorption , nuclear chemistry , organic chemistry , enzyme , physics , meteorology , nuclear physics
Recently the use of minced horseradish ( Armoracia rusticana P. Gaertner, Meyer & Scherb.) roots has been proposed for the decontamination of waters polluted with chlorinated phenols. In this study the horseradish treatment is further evaluated using water containing phenols and anilines. 2,4‐Dichlorophenol sorption by the plant tissues in the absence of H 2 O 2 ranged between 40% at pH 2 and 10% at pH 10. The adsorbed compound could easily be extracted with water and methanol. In the presence of H 2 O 2 , most 2,4‐dichlorophenol was polymerized and could be removed from the aqueous solution through precipitation or binding to horseradish. The activity of 1 g of minced horseradish roots was equivalent to 22 purpurogallin units of the purified horseradish peroxidase. Maximal precipitation of 2,4‐dichlorophenol was observed at pH values ranging from 5 to 7 and at initial substrate concentrations between 7 and 8 m M . Horseradish application resulted in 99% removal of 27 compounds among 50 compounds tested. These results indicate that the use of horseradish is a feasible treatment for waters contaminated with phenols and anilines.