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Concentrations of Coumestrol and 4′ ,7‐dihydroxyflavone in Four Alfalfa Cultivars after Exposure to Ozone
Author(s) -
Skärby L.,
Pell E. J.
Publication year - 1979
Publication title -
journal of environmental quality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 171
eISSN - 1537-2537
pISSN - 0047-2425
DOI - 10.2134/jeq1979.00472425000800030004x
Subject(s) - coumestrol , cultivar , ozone , biology , horticulture , agronomy , botany , chemistry , organic chemistry , genistein , endocrinology , daidzein
Ozone can induce increases of 4′ ,7‐dihydroxyflavone (4′ ,7‐DHF) but not coumestrol in the ‘Buffalo’ alfalfa cultivar. This study was designed to determine whether these responses were unique to ‘Buffalo’ or characteristic of other alfalfa cultivars. Seven‐week‐old seedlings of ‘Ladak,’ ‘Sonora,’ ‘Moapa,’ and ‘Vernal’ alfalfa cultivars were exposed to 773 µ g/m3 (0.40 ppm) ozone for 3 hours. Forty‐eight hours after exposure, all foliage exhibiting chiorosis to extensive necrosis were harvested. Similar‐aged foliage from non‐ozonized control plants was also harvested. Methanolic extracts of the tissue were prepared and the presence and quantity of coumestroi and 4′,7‐DHF determined. Coumestrol was not detected in control or ozonized tissue. Nonozonized tissue was free of detectable 4′,7‐DHF. Injured foliage of all four cultivars contained elevated levels of 4′,7‐DHF as high as 91 ppm. There were no significant differences in concentrations of 4′,7‐DHF when concentrations were compared in four ozonized cultivars.