Open AccessIsolation of a Potential Antifungal Protein Produced by Epichloë festucae , a Fungal Endophyte of Strong Creeping Red Fescue
Author(s) -
Tian Zipeng,
Wang Ruying,
Ambrose Karen V.,
Clarke Bruce B.,
Belanger Faith C.
Publication year - 2017
Publication title -
international turfgrass society research journal
Language(s) - English
Resource type - Journals
ISSN - 2573-1513
DOI - 10.2134/itsrj2016.08.0723
Subject(s) - endophyte , biology , festuca rubra , powdery mildew , botany , yeast , antifungal , epichloë , plant use of endophytic fungi in defense , isolation (microbiology) , microbiology and biotechnology , symbiosis , bacteria , biochemistry , genetics
Festuca rubra L. subsp. rubra (strong creeping red fescue) exhibits endophyte‐mediated fungal disease resistance. Epichloë festucae Leuchtm., Schardl & M.R. Siegel, the fungal endophyte of F. rubra subsp. rubra , produces an abundant transcript encoding a potential secreted antifungal protein. The objective of this work was to confirm the presence of the antifungal protein in the apoplastic proteins and to express it in the yeast Pichia pastoris for future tests for activity against turfgrass fungal pathogens.