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A Reliable Method for Processing Soybean Seed for Laboratory Analysis 1
Author(s) -
Schild J. A.,
Olson R.A.
Publication year - 1983
Publication title -
agronomy journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 131
eISSN - 1435-0645
pISSN - 0002-1962
DOI - 10.2134/agronj1983.00021962007500060041x
Subject(s) - grinding , glycine , dry ice , agronomy , serial dilution , contamination , dry weight , pulp and paper industry , mathematics , environmental science , chemistry , materials science , biology , engineering , metallurgy , composite material , medicine , ecology , biochemistry , alternative medicine , amino acid , pathology
Established procedures for milling soybean [ Glycine max (L.) Merr.] seed entail use of solvents which can be dangerous to the user of the mill and which may cause uncertain dilutions and undesirable changes in the seed. A method for dry milling soybean seed was developed in this study to process seed material for assay, including 15 N isotope ratio analysis. The method involved the use of a sealed grinding device and dry ice. The seed and ice are ground together for 2 min. The resulting meal is similar in texture and recoverability to that obtainable by grinding in a solvent, and the problem of cross contamination is minimal.