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Sodium Hypobromite Oxidation Method for Determination of Total Phosphorus in Plant Materials 1
Author(s) -
Dick W. A.,
Tabatabai M. A.
Publication year - 1982
Publication title -
agronomy journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 131
eISSN - 1435-0645
pISSN - 0002-1962
DOI - 10.2134/agronj1982.00021962007400010017x
Subject(s) - chemistry , phosphate , sodium , phosphorus , chromatography , digestion (alchemy) , nuclear chemistry , biochemistry , organic chemistry
A simple, sensitive, precise, and rapid method for determination of total P in plant materials is described. The sample is heated with alkaline sodium hypobromite solution to oxidize the P compounds to phosphate, and the phosphate thus obtained is extracted with 1 N H 2 SO 4 and determined colorimetrically by a heteropoly blue color method. Comparison of results obtained by the hypobromite oxidation and HNO 3 ‐HCIO 4 digestion procedures showed that the average total P values by the two methods for 20 plant samples and animal manures analyzed were 0.385 and 0.384%, respectively. In addition to being safe, digestion of plant samples with sodium hypobromite solution and colorimetric determination of the extracted P by the method of Murphy and Riley permits 100 analyses to be performed by a single operator in a normal working day.