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Use of Helminthosporium maydis Race T Pathotoxin to Determine Disease Reaction of Germinating Corn Seed 1
Author(s) -
Lim S. M.,
Hooker A. L.,
Smith D. R.
Publication year - 1971
Publication title -
agronomy journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 131
eISSN - 1435-0645
pISSN - 0002-1962
DOI - 10.2134/agronj1971.00021962006300050016x
Subject(s) - seedling , germination , bioassay , biology , zea mays , cytoplasm , agronomy , horticulture , botany , genetics
Several methods of pathotoxin production and corn ( Zea may L.) seedling bioassays are described as techniques to determine the disease susceptibility of corn to southern corn leaf blight. Toxin produced by race T of H. maydis is specific in inhibiting the elongation of primary roots of seedlings with T cms cytoplasm; primary roots of seedlings with normal cytoplasm are not so greatly inhihited. Filtrates obtained by several different methods from race T grown in culture all exhibited a similar pathotoxic specificity. Extracts obtained from corn leaves with T cms cytoplasm and infected with race T also produced the specific reaction in corn seedlings. The corn seedling bioassay with race T pathotoxin is a useful technique to determine the proportion of T cms and normal corn in any blended seed lot.