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Adaptation of Gas‐Liquid Chromatography to Analysis of Certain Sugars of Cotton Leaves 1
Author(s) -
Sabbe Wayne E.,
Cathey George W.
Publication year - 1970
Publication title -
agronomy journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 131
eISSN - 1435-0645
pISSN - 0002-1962
DOI - 10.2134/agronj1970.00021962006200010012x
Subject(s) - chemistry , silylation , chlorosilane , fructose , sucrose , chromatography , gas chromatography , sugar , sample preparation , organic chemistry , catalysis , silicon
A procedure involving gas‐liquid chromatography (GLC) of certain sugars in cotton leaves was studied. The use of hexamethyl‐disilazane (HMDS) and trimethyl‐chlorosilane (TMS) for silylation of fructose, glucose, and sucrose gave recovery percentages ranging from 71 to 105%. The substitution of trifluoracetic acid (TFA) for TMS gave recovery percentages of 91 to 109%. The use of TFA also improves the ease of sample preparation since absolute exclusion of water was not necessary. Details of sample preparation and operating parameters of GLC are given.