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Detection of Iron Ethylenediamine Di(O‐Hydroxyphenylacetate) in Plant Tissue 1
Author(s) -
Jeffreys R. A.,
Wallace A.
Publication year - 1968
Publication title -
agronomy journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 131
eISSN - 1435-0645
pISSN - 0002-1962
DOI - 10.2134/agronj1968.00021962006000060008x
Subject(s) - chemistry , ethylenediamine , chelation , extraction (chemistry) , ammonium acetate , environmental chemistry , chromatography , inorganic chemistry , high performance liquid chromatography
A method was developed for the extraction of iron ethylenediamine di(ο‐hydroxyphenylacetate) (FeEDDHA) from plant tissue and the technique was used to study the absorption of FeEDDHA by plants. A published method for the extraction of FeEDDHA was modified in an attempt to eliminate plant components which interfered with the spectrophotometric determination of the iron chelate. The method which was developed involves the use of lead acetate and ammonium sulfate to precipitate interfering substances. The FeEDDHA is then extracted into η‐amyl alcohol. Tobacco plants were exposed to FeEDDHA at various concentrations and for various periods of time. The leaves of the plants were subsequently analyzed to determine whether they contained detectable amounts of the intact iron chelate. The results suggested that FeEDDHA accumulated in the leaves of plants which had been grown on the high levels of the iron chelate or which had been grown on FeEDDHA for long periods of time.