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Standard Procedure for the Quantitative Determination of Individual Sugars and Total Soluble Carbohydrate Materials in Plant Extracts 1
Author(s) -
Nalewaja John D.,
Smith Lawrence H.
Publication year - 1963
Publication title -
agronomy journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 131
eISSN - 1435-0645
pISSN - 0002-1962
DOI - 10.2134/agronj1963.00021962005500060006x
Subject(s) - citation , library science , mathematics , operations research , computer science
TJRESENT-DAY agronomic research frequently requires ••quantitative determination of carbohydrate materials in plants. However, there is no standardized procedure in the literature to provide complete and detailed information covering sample preparation through final calculations. Carbohydrate extraction is accomplished by grinding plant materials in a Waring blender. Waldron et al. (4) found that grinding fresh tissue in a Waring blender was an effective method for extracting sugars from plant materials. Clarification and desalting of plant extracts required in procedures currently being used (2, 4, 5) are eliminated, largely because of the specificity of the phenol-sulfuric acid method for carbohydrate materials (1). The procedure of Dubois et al. (1) is modified and standardized making possible rapid quantitative determination of large numbers of individual sugars in aqueous and