Open AccessThe Antibacterial Activity of Dayak Onion (Eleutherine palmifolia (L.) Merr) towards Pathogenic Bacteria
Author(s) -
Tiara Dini Harlita,
Oedjijono Oedjijono,
Ari Asnani
Publication year - 2018
Publication title -
tropical life sciences research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.313
H-Index - 16
eISSN - 2180-4249
pISSN - 1985-3718
DOI - 10.21315/tlsr2018.29.2.4
Subject(s) - bacillus cereus , ethyl acetate , antibacterial activity , cereus , chemistry , staphylococcus aureus , food science , minimum inhibitory concentration , ethanol , bacteria , pathogenic bacteria , traditional medicine , biology , chromatography , antimicrobial , biochemistry , organic chemistry , medicine , genetics
Antibacterial activity of indigenous Dayak onion ( Eleutherine palmifolia (L.) Merr) was investigated. The Dayak onion was solvent extracted with n -hexane, ethyl acetate, and ethanol 96% consecutively. Each extract was tested its antibacterial activity towards methicillin-resistant Staphylococcus aureus (MRSA), Bacillus cereus , Shigella sp., and Pseudomonas aeruginosa using disc diffusion method. The test results showed that the n -hexane, ethyl acetate, and ethanol 96% extracts positively inhibited the growth of MRSA, B. cereus , Shigella sp., and P. aeruginosa . The highest inhibition activity of each extract was obtained with 10 mg/mL of extract concentration; whereas the minimum inhibitory concentration (MIC) of each extract was 2 mg/mL. Extract with the highest inhibition activity was ethyl acetate extract against B. cereus (139.58%). TLC evaluation of ethyl acetate extract showed four spots and bioautography indicated that ethyl acetate extract contained four types of compounds with inhibition activity against B. cereus , in which two compounds have higher antibacterial activity than the other two.