Open AccessExpression of Circulating MicroRNA-141 in Epithelial Ovarian Cancer
Author(s) -
Akbar Satria Fitriawan,
Aprilia Indra Kartika,
Siti Nur Chasanah,
Teguh Aryandono,
Sofia Mubarika Haryana
Publication year - 2020
Publication title -
the malaysian journal of medical sciences/the malaysian journal of medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.394
H-Index - 25
eISSN - 2180-4303
pISSN - 1394-195X
DOI - 10.21315/mjms2020.27.6.4
Subject(s) - microrna , ovarian cancer , epithelial ovarian cancer , cancer research , oncology , expression (computer science) , medicine , biology , cancer , gene , genetics , computer science , programming language
Background Epithelial ovarian cancer (EOC) is a lethal disease due to late diagnosis and lack of effective screening methods. MicroRNA (miR/miRNA) plays an important role in ovarian carcinogenesis and may serve as a non-invasive biomarker for EOC. This study aimed to assess miR-141 expression in the blood plasma of patients with EOC and healthy subjects and determine its association with the clinical stage of EOC. Methods This cross-sectional study used blood plasma from 30 newly diagnosed untreated patients with EOC and 25 healthy subjects. The mean age was 47.73 (SD = 10.29) years for EOC and 44.48 (SD = 16.14) years for healthy subject. The total RNA was isolated from blood plasma and reversed transcribed to obtain cDNA. The expression of miR-141 was measured by real-time quantitative polymerase chain reaction (qRT-PCR), and calculated using 2 −ΔΔCt methods. The data were analysed using Mann-Whitney test. Results The expression of miR-141 was upregulated 8.41 fold in the blood plasma of EOC patients compared to healthy controls ( P < 0.001). Expression of miR-141 in the advanced stage was upregulated 4.2 fold compared to the early stage ( P < 0.001). Conclusion The miR-141 was upregulated in the blood plasma of EOC and associated with an advanced stage of disease, suggesting it has potential as a biomarker for EOC detection.