Open AccessViability and DNA Damage of Buccal Mucosa Cells in Patients Exposed to Panoramic X-ray
Author(s) -
Ryna Dwi Yanuaryska,
Afit Aditya Atmoko,
Isti Rahayu Suryani,
Ratna Shantiningsih
Publication year - 2021
Publication title -
archives of orofacial sciences/archives of orofacial science
Language(s) - English
Resource type - Journals
eISSN - 2231-7163
pISSN - 1823-8602
DOI - 10.21315/aos2021.16.s1.8
Subject(s) - comet assay , viability assay , trypan blue , dna damage , genotoxicity , buccal swab , buccal administration , pathology , microbiology and biotechnology , biology , cell , medicine , andrology , chemistry , dna , toxicity , dentistry , genetics
Panoramic X-ray is well known to cause DNA damage and induces cellular death. The aim of the present study was to evaluate the cytotoxicity of radiation exposure from panoramic radiography on human buccal mucosa cells by assessing the cell viability using the simple-trypan blue exclusion test. The genotoxicity effect was evaluated by assessing comet assay score. This research included a total of 20 healthy patients who had panoramic radiography for a routine dental examination. Buccal mucosa cells were collected from all participants before X-ray exposure and at 30 min or 24 h after exposure in Groups 1 and 2, respectively, and subjected to a comet assay and trypan blue exclusion test to assess cell viability and DNA damage. Cell viability was calculated as the ratio of live (translucent) to total counted cells. Comet assay output images were analysed using OpenComet software and a visual score by measuring the percentages of tail DNA and summing the visual score, respectively. A statistically significant (p 0.05). The results showed that panoramic radiography interfered cell viability and induced DNA damage in buccal mucosa cells within 30 min after exposure, but these effects were ceased after 24 h.