Open AccessThe new attenuated strain of <i>Mycobacterium tuberculosis</i> BN. Characteristics and vaccine properties
Author(s) -
Б. В. Никоненко,
Tatyana Azhikina,
Artem Grigorov,
Irina Linge,
Н. Н. Логунова,
S. I. Kayukova
Publication year - 2021
Publication title -
tuberkulez i bolezni lëgkih/tuberkulëz i bolezni lëgkih
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.226
H-Index - 12
eISSN - 2542-1506
pISSN - 2075-1230
DOI - 10.21292/2075-1230-2021-99-10-60-65
Subject(s) - virulence , strain (injury) , mycobacterium tuberculosis , biology , microbiology and biotechnology , virology , attenuated vaccine , inbred strain , tuberculosis vaccines , gene , tuberculosis , genetics , medicine , pathology , anatomy
The objective of the study: to obtain a live attenuated strain and investigate its properties by multiple cultures of the virulent strain of Mycobacterium tuberculosi s H37Rv. Subjects and Methods. The original virulent strain H37Rv was subcultured 70 times in 7H9 liquid medium. Genetic properties of the new strain, degree of avirulence, and vaccine properties were studied. Results. Mycobacteria of the new strain Mtb BN lost their virulence to inbred mice. Eight mutations were identied by whole genome sequencing: single nucleotide insertions and deletions (in/del) distinguishing the Mtb BN and H37Rv strains. The Mtb BN strain demonstrated vaccine potential at the BCG level. Additionally, in some genetic models, the attenuated strain was highly eective in protecting inbred mice when infected with Mtb H37Rv as opposed to BCG.