Open AccessGenetic Analysis of 11 Polymorphic Isozyme Loci in Chestnut Species and Characterization of Chestnut Cultivars by Multi-locus Allozyme Genotypes
Author(s) -
Hongwen Huang,
Fenny Dane,
J. D. Norton
Publication year - 1994
Publication title -
journal of the american society for horticultural science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.408
H-Index - 80
eISSN - 2327-9788
pISSN - 0003-1062
DOI - 10.21273/jashs.119.4.840
Subject(s) - biology , locus (genetics) , isozyme , intraspecific competition , mendelian inheritance , interspecific competition , genetics , genotype , botany , gene , zoology , enzyme , biochemistry
Allozyme polymorphism in chestnut ( Castanea ) species was investigated using isoelectric focusing in thin-layer polyacrylamide slab gels. Genetic analysis of the progenies of intraspecific crosses and interspecific F 2 s and backcrosses (BC 1 s) allowed the verification of 11 polymorphic isozyme loci from 11 enzyme systems. The following loci were defined: Acp, Adh, Est-1, Est-2, Est-5, Me, Prx-1, Prx-2, Prx-3, Skd-3, and Skd-4 . All polymorphic loci behaved as single-locus Mendelian genes. Skd showed unique species specificity. Skd-1 and Skd-2 were unique to the American chestnut ( C. dentata Borkh.) and the European chestnut ( C. sativa Mill.), whereas Skd-3 and Skd-4 were unique to the Chinese chestnut ( C. mollissima Bl.) and the Japanese chestnut ( C. crenata Sieb.). Linkage analysis revealed linkage for three pairs of loci: Skd-3 / Skd-4 , Est-1 / Est-2 , and Est-5 / Prx-1 . The single-tree progeny method was used successfully for isozyme genetic analysis. Forty-seven chestnut cultivars in six chestnut species were characterized using 12 isozyme loci and can be unambiguously identified by 12 multi-locus genotypes. The interspecific and geographic relationships among species were also discussed.