Open AccessHuman γδ T Cells Augment Antigen Presentation in Listeria Monocytogenes Infection
Author(s) -
Yuli Zhu,
Huaishan Wang,
Yi Xu,
Yu Hu,
Hui Chen,
Liyuan Cui,
Jianmin Zhang,
He Wang
Publication year - 2016
Publication title -
molecular medicine
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.844
H-Index - 118
eISSN - 1528-3658
pISSN - 1076-1551
DOI - 10.2119/molmed.2015.00214
Subject(s) - listeria monocytogenes , biology , antigen presentation , immune system , microbiology and biotechnology , antigen , antigen presenting cell , cd14 , cytotoxic t cell , t cell , immunology , in vitro , bacteria , biochemistry , genetics
Circulating γδ T cells in healthy individuals rapidly respond to bacterial and viral pathogens. Many studies have demonstrated that γδ T cells are activated and expanded by Listeria monocytogenes ( L.monocytogenes ), a foodborne bacterial pathogen with high fatality rates. However, the roles of γδ T cells during L.monocytogenes infection are not clear. In the present study, we characterized the morphological characteristics of phagocytosis in γδ T cells after L.monocytogenes infection using transmission electron microscopy. Results show activation markers including HLA-DR and lymph node-homing receptor CCR7 on γδ T cells were upregulated after stimulation via L.monocytogenes . Significant proliferation and differentiation of primary αβ T cells was also observed after co-culture of peripheral blood mononuclear cells with γδ T cells anteriorly stimulated by L.monocytogenes . L.monocytogenes infection decreased the percentage of γδ T cells in mouse IELs and increased MHC-II expression on the surface of γδ T cells in vivo . Our findings shed light on antigen presentation of γδ T cells during L.monocytogenes infection.