Open AccessROLE OF MEDIUM SUPPLEMENTATION OF GROWTH MODULATORS TO ALLEVIATE CADMIUM-INDUCED OXIDATIVE STRESS IN MAIZE
Author(s) -
Azka Javaid
Publication year - 2019
Publication title -
pakistan journal of agricultural sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.22
H-Index - 20
eISSN - 2076-0906
pISSN - 0552-9034
DOI - 10.21162/pakjas/19.8406
Subject(s) - ascorbic acid , toxicity , oxidative stress , chemistry , proline , reactive oxygen species , food science , glutathione , salicylic acid , shoot , osmoprotectant , antioxidant , cadmium , biochemistry , growth inhibition , botany , biology , amino acid , enzyme , organic chemistry , apoptosis
Cadmium (Cd) toxicity has been a wide-spread phenomenon affecting plant metabolic functions due to excessive production of reactive oxygen species. This study was aimed at medium supplementation of optimized levels of growth modulators viz., salicylic acid (SA, 500 µM), ascorbic acid (AsA, 500 µM), thiourea (TU, 400 µM) and hydrogen peroxide (H2O2, 100 µM) in improving Cd tolerance in maize (Zea mays L. hybrid P-1543) in sand culture under net house conditions. All treatments were applied in the growing medium by dissolving in water. Results revealed that Cd stress (1000 µM) severely reduced the shoot and root dry mass and leaf area per plant and endogenous production of osmoprotectants (soluble sugars, free proline and glycinebetaine), phenolic compounds (soluble phenolics, flavonoids and anthocyanins) and vitamins (ascorbic acid, niacin and riboflavin) in shoot and root tissues indicating that Cd was highly toxic to the metabolic pathways. However, medium supplementation of the selected growth modulators was quite effective in reversing the Cd toxicity effect. Among the medium supplemented compounds, SA and AsA were more effective in reversing the Cd toxicity effect than TU and H2O2. Presence of tight correlations of growth attributes with metabolites levels substantiated that medium supplementation was greatly effective in orchestrating the endogenous metabolite levels and supporting maize growth under Cd toxicity. A greater effectiveness of the SA, AsA and TU can be attributed to their molecular structure and physiological properties in scavenging ROS and reducing oxidative stress. The results may have great promise and practical implications for growing maize successfully in the marginally Cd contaminated soils.