Open AccessBACTERIAL AND PCR BASED DIAGNOSIS OF NATURALLY OCCURRING BOVINE TUBERCULOSIS IN CATTLE AND BUFFALOES
Author(s) -
Anna Jaroszewska
Publication year - 2019
Publication title -
pakistan journal of agricultural sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.22
H-Index - 20
eISSN - 2076-0906
pISSN - 0552-9034
DOI - 10.21162/pakjas/19.6608
Subject(s) - tuberculosis , mycobacterium bovis , herd , bovine tuberculosis , tuberculin , veterinary medicine , ziehl–neelsen stain , biology , mycobacterium tuberculosis , mastitis , polymerase chain reaction , dairy cattle , medicine , zoology , microbiology and biotechnology , sputum , pathology , acid fast , biochemistry , gene
The aim of the study was to determine the effect of the effective organisms (EM) and the granulated manure on the chemical properties of Japanese knotweed (Reynoutria japonica Houtt.) and the possibility of using them as a component of functional food. The field experiment was carried out in 2015-16 at the Lipnik Experimental Station (53°12'N; 14°27'E), Poland. The experiment consisted of four objects in four replications: control, effective microorganisms – EM, manure, effective microorganisms + manure. In the experiment, EmFarma PlusTM was used, which contained a mixture of specially selected non-genetically modified strains of microorganisms and their metabolites in the fermented mixture of natural ingredients. The granulated manure contained N – 2.1, P2O5 – 1.6, K2O – 5.9, Ca – 2.0, Mg 0.5 (% DM), organic substances (60% DM), and humic substances (25% DM). Compared with the control the EM, manure, and EM+manure study groups had significantly higher amounts of crude fat, by 17.8, 0.8 and 15.7 g.kg-1 , respectively. The EM and EM+manure groups had significantly higher amounts of crude ash, by 7.4 and 16 g.kg-1 , respectively. The highest phosphorus levels were observed in the EM group by 0.62 g.kg-1 . The EM and EM+manure groups had significantly higher amounts of potassium, by 3.8 g.kg-1 . The higher amounts of calcium (by 1.52 g.kg-1 ) had the EM+manure group. The EM, manure and EM+manure study groups had significantly higher amounts of zinc (6.5, 7.2, 17.6 mg.kg-1 , respectively), iron (2.1, 2.0, 14.1 mg.kg-1 , respectively) and manganese (16.4, 49.6, 16.0 mg.kg-1 , respectively). The highest molybdenum levels were found in the group fertilized EM and manure, 4.5 and 5.5 mg.kg-1 , respectively. Due to the high nutrient content, leaves of Japanese knotweed can be used as an alternative source of components in the production of functional foods and pharmaceutical preparations.