Open AccessA Fast Dual-responsive OFF-ON Fluorescent Probe for Cysteine and Glutathione without Interference from Homocysteine
Author(s) -
Qingjie Fu,
Ruxin Liu,
Xiaoqian Cui,
Yuqi Zhao,
Hui Chi,
Yongzhuang Lu,
Qiang Fei,
Guodong Feng,
Hongyan Shan,
Yanfu Huan
Publication year - 2021
Publication title -
analytical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 73
eISSN - 1348-2246
pISSN - 0910-6340
DOI - 10.2116/analsci.21p088
Subject(s) - chemistry , glutathione , cysteine , fluorescence , homocysteine , interference (communication) , biophysics , biochemistry , enzyme , physics , channel (broadcasting) , quantum mechanics , electrical engineering , biology , engineering
Abnormal levels of biothiols, such as cysteine (Cys), homocystine (Hcy), and glutathione (GSH), are generally known to result in various diseases. A fast dual-responsive OFF-ON fluorescent probe HBO-AC was synthesized and developed. Non-fluorescent HBO-AC can sense Cys by regaining fluorescence at 444 nm within 10 min and a response to GSH by restoring fluorescence at 349 nm within 20 min. There is no mutual interference with Δλ ca. 100 nm. A novel method was developed by utilizing a low reaction rate between HBO-AC and Hcy to eliminate common interference from Hcy. A successful determination of Cys and GSH in fetal bovine serum (FBS) indicated that the probe had potential application for clinical diagnosis. Moreover, it was confirmed that HBO-AC can resist interference from protein to some extent, since FBS was not pretreated before use.