Open AccessTLC-based MS Imaging Analysis of Glycosphingolipids and Glycerin Fatty Acid Esters after 1,2-Dichloroethane Washing
Author(s) -
Shoko Matsushita,
Takuma Hasegawa,
Marina Hiraoka,
Aki Hayashi,
Yusuke Suzuki
Publication year - 2021
Publication title -
analytical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 73
eISSN - 1348-2246
pISSN - 0910-6340
DOI - 10.2116/analsci.21c009
Subject(s) - chemistry , chromatography , thin layer chromatography , dichloroethane , membrane , mass spectrometry , polyvinylidene fluoride , organic chemistry , biochemistry , catalysis
Matrix-assisted laser desorption/ionization-based mass spectrometry imaging (MSI) of separated lipids on thin-layer chromatography (TLC) plates or followed by blotted hydrophilic polyvinylidene fluoride (PVDF) membranes has become a powerful tool in lipidomic analyses. However, background peaks in MS spectra often cover lipid peaks in a low amount/ionization effect; consequently, only low intensities/resolutions MSI are obtained. To address the aforementioned problem, we attempted 1,2-dichloroethane pre-washing of TLC plates before development and found that backgrounds could successfully be removed from the TLC plate or PVDF membrane.