Open AccessRelationship between the Macrophage Activity with Interleukin-6 Levels and Titers of Antibodies against Salmonella typhi
Author(s) -
I Nengah Kerta Besung,
Nyoman Mantik Astawa,
Ketut Suata,
Ni Ketut Suwiti
Publication year - 2016
Publication title -
jurnal kedokteran hewan
Language(s) - English
Resource type - Journals
eISSN - 2502-5600
pISSN - 1978-225X
DOI - 10.21157/j.ked.hewan.v10i1.3359
Subject(s) - salmonella typhi , titer , macrophage , antibody , antibody titer , salmonella , microbiology and biotechnology , immunology , typhoid fever , biology , bacteria , biochemistry , in vitro , gene , genetics , escherichia coli
This study aims to determine the relationships between macrophage activities and interleukin-6 (IL-6 levels), and titer antibody to Salmonella typhi (S. typhi). A total of 32 mice were infected intraperitoneally with 105 cells/ml of S. typhi. The activities of macrophages wereobserved a day after the infection, while the observations of the levels of IL-6 and titer antibody employing enzyme-linked immunosorbent assay(ELISA) method were conducted after one and two weeks of infection. The results showed that the average activities of macrophages afterinfected by S. typhi was 77.84±11.23 sel, and the levels of IL-6 and antibody titers were higher (159.55±90.11 pg and 56.87±23.32 IUrespectively). The macrophage activities were significantly increased (P<0.01) with increasing the level of IL-6 to Y= -0.317x494.09 with the relatedness (r)= 0.89 and the antibody titers to Y= -0.020x2 + 0.00031x + 29.083 with the relatedness (r)= 0.95.Key words: macrophage activity, IL-6, antibody titers, Salmonella typhi2 + 0.0033x2 +