Open AccessKinetic studies of AST isoenzymes I,II,III,IV partially purified from patient,s urine with chroinc renal failure
Author(s) -
Baghdad Science Journal
Publication year - 2010
Publication title -
mağallaẗ baġdād li-l-ʿulūm
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.167
H-Index - 6
eISSN - 2411-7986
pISSN - 2078-8665
DOI - 10.21123/bsj.7.1.437-443
Subject(s) - urine , michaelis–menten kinetics , isozyme , q10 , chemistry , arrhenius equation , substrate (aquarium) , chromatography , enzyme , nuclear chemistry , biochemistry , medicine , enzyme assay , biology , activation energy , ecology , respiration , anatomy
In this research, the kinetic studies of four isoenzymes of Asprtate aminotransferase, which partially purified from the urine of chronic renal failure patients were carried out .The four isoenzymes were obeyed Michaelis-Menton's equation and the optimum concentration of their substrate (Aspartic acid) was (166.5x10-3) mole/liter,and their Km values were determined. Four isoenzymesI,II,III,IV have shown an optimum pH at 7.4.The four isoenzymes obeyed Arrhenius equation up to 37º C and their Ea and Q10 constants were determined .