Open AccessPurification and Characterization β - lactamase produce from local isolate Klebsiella pneumonia
Author(s) -
Baghdad Science Journal
Publication year - 2009
Publication title -
baghdad science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.167
H-Index - 6
eISSN - 2411-7986
pISSN - 2078-8665
DOI - 10.21123/bsj.6.1.50-60
Subject(s) - size exclusion chromatography , chromatography , chemistry , enzyme , ammonium , ammonium sulfate precipitation , klebsiella pneumonia , filtration (mathematics) , precipitation , microbiology and biotechnology , biochemistry , biology , escherichia coli , gene , mathematics , statistics , physics , meteorology , organic chemistry
Beta-lactamase was purified from local isolate Klebsiella pneumonia by several steps included precipitation with ammonium sulphate at 20-40% saturation, DEAE- ion exchange chromatography and gel filtration on Sephacryl S-200 column. The obtained purification fold and recovery were 32.66; 47.04% respectively. The characterization of the purified beta-lactamase showed that the molecular weight was about 4000 daltons as determined by gel filtration.Purified enzyme had an optimal pH of 7 for activity and an optimal stability between pH 6.5-7.5, results shows that the optimal temperature appear to be 35 ? C .During storage the enzyme retained 72% at -20 ? C and retained 25% of the activity at the same period at 4 ? C.
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