Open AccessDetermination Of Micro Amount Of Spironolactone In Some Of Pharmaceutical Preparate By Using a Molecular Luminescence Technique.
Author(s) -
Baghdad Science Journal
Publication year - 2008
Publication title -
mağallaẗ baġdād li-l-ʿulūm
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.167
H-Index - 6
eISSN - 2411-7986
pISSN - 2078-8665
DOI - 10.21123/bsj.5.1.55-62
Subject(s) - molar absorptivity , analytical chemistry (journal) , detection limit , relative standard deviation , chemistry , calibration curve , chromatography , luminescence , mathematics , materials science , optics , physics , optoelectronics
The present study include a new developed method of analysis for determination of drug Spironolaction (SP) in some Pharmaceuticals by Spectrofluorometric method.Spironolaction was determined under optimal experimental condition that follows :-The excitation spectrum was (l=351 nm), the emmetion spectrum was (l=518 nm), pH=1, the suitable temperature for reaction 60oC and the optimal time less than (3) minute. The analysis and rang statistical data was:-Linear dynamic rang (1-10) ?g.ml-1, the detection limit (D.L = 0.023 ?g.ml-1), Molar absorptivity (? = 29875 liter mole-1 cm-1), Relative standard deviation (%RSD = 0.78), (%Erel = 3.3) and recovery (Rec = 96.6) percentage.Determination of Spironolactone was accomplished by two methods(A) Using direct calibration curve method, Corr. Coef. (R = 0.988 and %Rec = 97.4).(B) Using standard additional methods Corr. Coef (R =0.988 and %Rec= 96.6).