Open AccessExtraction and Purification of Indole aectic acid from locale isolate Fusarium oxysporum(F2)
Author(s) -
Essam F. A. Al-Juamily,
Nikhat Ahmed,
Ali O. A. Al-Shimary
Publication year - 2008
Publication title -
mağallaẗ baġdād li-l-ʿulūm
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.167
H-Index - 6
eISSN - 2411-7986
pISSN - 2078-8665
DOI - 10.21123/bsj.5.1.27-37
Subject(s) - fusarium oxysporum , chromatography , high performance liquid chromatography , absorbance , acetic acid , column chromatography , extraction (chemistry) , chemistry , indole 3 acetic acid , indole test , silica gel , ethyl acetate , botany , biology , organic chemistry , biochemistry , auxin , gene
Indole acetic acid (IAA) produced from F. oxysporum (F2) was purified by several steps included extraction by cold ethyl acetate ; Column chromatography using silica gel and TLC chromatography .The pure indole acetic acid (IAA) which produce by F. oxysporum (IAA) was tested by ultraviolet spectra at (200-300)nm ; and appear that the maximum absorbance at 229nm , the high performance liquid chromatography (HPLC) used to test the purity of the indole acetic acid and the results showed one peak at appearance time 3.822 min