Open AccessEffect of the p38 MAPK Inhibitor on the Expression of Metalloproteinases and Their Inhibitors during the Formation of Abdominal Adhesions
Author(s) -
И. А. Шурыгина,
Л. В. Родионова,
Natalia Ayushinova,
Елена Чепурных,
И. С. Трухан,
М. Г. Шурыгин
Publication year - 2021
Publication title -
international journal of biomedicine
Language(s) - English
Resource type - Journals
eISSN - 2158-0529
pISSN - 2158-0510
DOI - 10.21103/article11(4)_oa9
Subject(s) - mmp2 , matrix metalloproteinase , mmp9 , mapk/erk pathway , p38 mitogen activated protein kinases , collagenase , abdominal cavity , intraperitoneal injection , adhesion , kinase , chemistry , pharmacology , medicine , gene , biology , microbiology and biotechnology , surgery , downregulation and upregulation , biochemistry , enzyme , organic chemistry
Background: The aim of this study was to assess the effect of blockade of the p38 mitogen-activated protein kinase (MAPK) on the expression of genes encoding metalloproteinases (MMPs) during the formation of adhesions in the abdominal cavity. Methods and Results: The experiments were carried out on male Wistar rats (n=75). The studies were carried out in two groups: Group 1 (control, n=35) – modelling the adhesive process; Group 2 (experimental, n=35) – modelling the adhesive process with intraperitoneal administration of Seroguard®—a prolonged form of the p38 MAPK inhibitor. The expression of the MMP1a, MMP2, MMP7, MMP9, and TIMP genes was assessed using real-time PCR. In the control group, overexpression of the MMP1a and MMP7 genes began from 6 hours after modeling the adhesive process, MMP9 – from Day 1, MMP2 – from Day 7 and persisted until the end of observation. With local blockade of p38 MAPK, the level of overexpression of genes encoding MMPs in the early stages was higher than in the control group (MMP1a – by Day 1; MMP7 – by 6 hours and Day 1, MMP9 – by 12 hours). From Day 3 to Day 14, the MMP1a and MMP7 expression in the experimental group was significantly lower than in the control group. Conclusion: The performed study demonstrated the involvement of different types of MMPs—collagenases (MMP1a), gelatinases (MMP2 and 9), matrilysins (MMP7)—in the rearrangement of the extracellular matrix during the process of adhesion formation in the abdominal cavity.