Open AccessThe neuroattenuation of three clone viruses isolated from the attenuated CTN rabies virus
Author(s) -
Leitai Shi,
Yongxin Yu,
Yuhua Li
Publication year - 2016
Publication title -
journal of applied virology
Language(s) - English
Resource type - Journals
eISSN - 2306-6210
pISSN - 2305-5154
DOI - 10.21092/jav.v5i3.77
Subject(s) - virology , rabies , rabies virus , rabies vaccine , virulence , biology , attenuated vaccine , virus , clone (java method) , guinea pig , duck embryo vaccine , reversion , microbiology and biotechnology , gene , biochemistry , phenotype , endocrinology
Three attenuated rabies virus strains(CTN181-3CTN181-16 and CTN181-19), were selected by plaque cloning from CTN-181 rabies attenuated viruswhich was infected guinea pigs submandibular glands.After passaging those viruses in suckling mice brain for five consecutive passages or in submandibular gland of guinea pigs for one passage, non-virulence reversion or increase were detected.The neuroattenuation stability make the three newly selected CTN attenuated strains the promising live vaccine candidates for wild animal prophylactic vaccination and even postexposure prophylaxis of rabies for humans.