Open AccessRabies virus-like particles comprised of G and M proteins protect BALB/c mice from lethal dose challenging
Author(s) -
Xiong Liu,
Huang Xiao-yuan,
Xiaoming Yang
Publication year - 2015
Publication title -
journal of applied virology
Language(s) - English
Resource type - Journals
eISSN - 2306-6210
pISSN - 2305-5154
DOI - 10.21092/jav.v4i2.30
Subject(s) - rabies virus , virology , rabies , vero cell , titer , rabies vaccine , duck embryo vaccine , recombinant dna , virus , neutralizing antibody , biology , gene , biochemistry
Virus-like particles (VLPs) were super-molecular aggregates without any replicative genetic information, so they have been suggested to be a promising vaccine approach with several commercial practices. In this study, we tried to constructed rabies VLPs with recombinant baculovirus contained G and M proteins from CVS strain of rabies virus. Expression products can be easily purified by ultrafiltration and gradient centrifugation, and then identified with ELISA, West-blotting and TEM, respectively. A continuous high serum neutralizing antibody titers extended weeks had been measured with RFFIT, after mice primary immunized with rabies VLPs and boost 7 days later. Furthermore, Similar to same titers inactivated rabies vaccine purified from Vero cell, nearly 100% protection from lethal CVS strains and another wild type strains can be observed 7 days after boost. Taken together, these finding indicates that rabies VLPs deserved as a more effective, flexible, costly and safer vaccine candidates.