Open AccessAnalisis Keragaman Genetik 161 Aksesi Mangga Indonesia Menggunakan Marka Mikrosatelit
Author(s) -
Tasliah Tasliah,
Habib Rijzaani,
Tri Hariyadi,
Siti Yuriyah,
Rebin Rebin,
Ma’sumah Ma’sumah,
Tiur S. Silitonga
Publication year - 2016
Publication title -
jurnal agrobiogen/jurnal agrobiogen
Language(s) - English
Resource type - Journals
eISSN - 2549-1547
pISSN - 1907-1094
DOI - 10.21082/jbio.v9n3.2013.p125-134
Subject(s) - upgma , microsatellite , germplasm , horticulture , genetic diversity , genotyping , biology , allele , genotype , zoology , genetics , population , medicine , gene , environmental health
Mango is one of the five important fruit crops in the world. Microsatellite markers can be used to analyze genetic diversity among mango accessions. The purpose of this research was to determine the relationship among mango germplasm collection using microsatellite markers. A total of 161 mango accessions originated from Indonesian Tropical Fruit Research Institute (Cukurgondang Field Station), Pasuruan, East Java, were used in this research. Twenty-six microsatellite markers were used to genotype each accession. Genotyping was conducted using Beckman Coulter® CEQ™ 8000 machine. Genetic relationship among accecions was analyzed using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) method, followed by bootstrap analysis. The result showed that high allele variation (15-75 alleles) was observed among mango accesions tested, with an average allele number of 38.69. The average of Polymophism Information Content (PIC) value was 0.548 (0.021-0.949). Fifteen microsatellite markers showed PIC value >0.5 indicated that these markers were suitable for mango diversity studies. Cluster analysis divided the mango collections into two groups. Group I consisted of 95 accessions, and group II consisted of 66 accessions. Ninety Indonesian indigenous mangos (84.11% of Indonesian mango accessions) could be separated from the introduced accessions.