Open AccessIdentifikasi Perubahan Karakter Agronomis Padi Transgenik Penanda Aktivasi cv. Asemandi Generasi T1
Author(s) -
Atmitri Sisharmini,
Aniversari Apriana,
Diah Nurmaliki,
Tri Joko Santoso,
Kurniawan Rudi Trijatmiko
Publication year - 2016
Publication title -
jurnal agrobiogen/jurnal agrobiogen
Language(s) - English
Resource type - Journals
eISSN - 2549-1547
pISSN - 1907-1094
DOI - 10.21082/jbio.v9n3.2013.p107-116
Subject(s) - genetically modified rice , biology , horticulture , transgene , chemistry , genetically modified crops , gene , biochemistry
The activation-tagged populations of transgenic rice cv. Asemandi have been developed by introducing construct of activation taq Ac/Ds into rice genom of cv. Asemandi. The T1 transgenic rice populations cv. Asemandi containing construct of activation taq have been obtained and needed to be characterized. This study aimed to identify the Basta herbicide resistant plants, transgenic plants containing hpt and bar genes, and changes in agronomic traits. Basta resistant plant was identified by treating leaf with basta solution. Hpt and bar genes were detected by PCR using specific primers. Phenotype characters were identified by observing and measuring their agronomic parameters. The study results showed that out of 315 rice transgenic cv. Asemandi T1 treated with Basta solution, 176 (55.87%) plants were indicated to be resistant to Basta. The results of PCR analysis revealed that eight rice transgenic cv. Asemandi tested contained both hpt and bar genes. In general, compared to the nontransgenic plants, there were changes in several agronomic parameters of T1 transgenic plants cv. Asemandi, including plant height, days to flowering, days to harvesting, periods of grain filling, and weight of 100 grains. Correlation analysis showed that there was no correlation between days of harvesting to weight of 100 grains in transgenic rice, but there was correlation in nontransgenic rice. Transgenic rice plants cv. Asemandi with changes in the agronomic characters will be useful for further study, such as to analyze the function of the genes.