Open AccessIdentifikasi Molekuler Hawar Daun Bakteri (Xanthomonas oryzae pv. oryzae) dan Uji Patogenisitasnya pada Galur-galur Padi Isogenik
Author(s) -
Tasliah Tasliah,
Mahrup Mahrup,
Joko Prasetiyono
Publication year - 2016
Publication title -
jurnal agrobiogen/jurnal agrobiogen
Language(s) - English
Resource type - Journals
eISSN - 2549-1547
pISSN - 1907-1094
DOI - 10.21082/jbio.v9n2.2013.p49-57
Subject(s) - xanthomonas oryzae , primer (cosmetics) , xanthomonas oryzae pv. oryzae , biology , pathogenicity , bacteria , microbiology and biotechnology , chemistry , genetics , organic chemistry
Identification of Xanthomonas oryzae pv. oryzae (Xoo) based on molecular analysis has been introduced just few years ago. This method used some specific primers for Xoo and can be done quickly. The purposes of this research were to identify isolate Xoo originated from five locations in Indonesia and to determine the level of pathogenicity of these bacteria. Studies were conducted in the greenhouse and the Molecular Biology Laboratory of ICABIOGRAD, from 2011 to 2012. Bacterial isolates were taken from five regions in Indonesia, namely: West Sumatra, West Java, Central Java, South Sulawesi, and West Kalimantan. The specific primers of Xoo were Xoo2967, Xoo80, and Xoo. Results showed that 216 isolates could be grown to form yellow colored colonies, which belongs to a criterian for Xoo. Molecular analysis demonstrated that 189 isolates were Xoo and 27 isolates were not. Amplification of DNA of the isolates resulted a 337 bp PCR product for primer Xoo2976, 700 bp for primer Xoo80 and 534 bp for primer Xoo. Pathogenicity tests of the Xoo isolates showed xa5, Xa7, and Xa21 resistance genes were still effective againts BLB pathogens originated from those five regions, with percentage of resistance were 93.57, 77.49, and 85.37%, respectively.