Transformasi Genetik Pisang Ambon dengan Gen Kitinase dari Padi

One of the main constrains on the productivity and quality enhancement of banana is wilt diseases caused by Fusarium oxysporum (Foc). Production decrease by wilt disease was 63.33%. Therefore, an effort to obtain the banana new variety which is tolerant to fusarium was absolutely necessary to be done. Genetic engineering can be used in new variety improvement, especially for production of pest and disease tolerant varieties. Transformation of banana with chi gene which expressed chitinase enzyme have been used in obtaining the plant resistant to Foc. The goals of the research were to obtain: determine lowest higromisin consentration inhibited nodul growth by tested four consentration of higromisin, determine optimum cocultivation time by tested three times cocultivation, tested asetosiringone added on two times cocultivation, and gen chi introduction at banana transforman shoots with PCR. The explants used were nodule induced from pseudostem of banana cv. Ambon kuning. Genetic transformation done by sowing the explants in bacterial suspension 0, 15, 30, and 45 minutes.The effect of asetosiringone (0 and 100 mg/l) on cocultivation medium was observed. The research results showed that the lowest higromisin concentration inhibited nodule growth was 25 mg/l for 5 weeks and the best time for inoculation of nodule were 30 minute. Asetosiringone added on bacterial suspension did not increase transformation efficiency. Chitinase gene transformation using Agrobacterium tumefaciens on banana nodules produced 25 noduly ines of putative transformant on selection media and 34 plants transforman identification by PCR.