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Keragaman Genetik 96 Aksesi Plasma Nutfah Padi Berdasarkan 30 Marka SSR Terpaut Gen Pengatur Waktu Pembungaan (HD Genes)
Author(s) -
Dwinita Wikan Utami,
Sutoro Sutoro,
Nurul Hidayatun,
Andari Risliawati,
Ida Hanarida
Publication year - 2016
Publication title -
jurnal agrobiogen/jurnal agrobiogen
Language(s) - English
Resource type - Journals
eISSN - 2549-1547
pISSN - 1907-1094
DOI - 10.21082/jbio.v7n2.2011.p76-84
Subject(s) - germplasm , biology , microsatellite , genetic diversity , genetics , allele , gene , marker assisted selection , genetic marker , horticulture , population , medicine , environmental health
Genetic Diversity of 96 Accession of Rice Germplasm Using 30 SSR Markers Linked to Heading Date Genes (HD Genes). Dwinita W. Utami, Sutoro, Nurul Hidayatun, Andari Risliawati, and Ida Hanarida. Rice with early maturity is one of an important genetic resources in rice germplasm collection. Characterization and identification of genetic diversity is an important issue for plant variety protection. Molecular identification by microsatellite markers using Genetic Analyzer enables resolve of this issue. The objective of this research is to identify the genetic diversity of 96 rice accessions based on their specific DNA fingerprint using microsatellite markers. A total of 96 accessions consisting of a diverse variety of maturity classification were genotyped with 30 SSR markers linked to HD genes which spread out in 12 chromosomes of rice geneome. The total 297 alleles were detected indicated the level of marker informativeness. RM5607 generated 7 allele with the size range from 103 to 197 and the highest PIC at 0.90. RM3571 (linked to HD12 gene) has a significant value associated with varieties which have early maturity trait. Clustering analysis showed the cluster based on Sub Species genome background and on early maturity trait.

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