Open AccessPOTENSI EKSTRAK DAUN PALIASA (Kleinhovia hospita) SEBAGAI ANTI Plasmodium falciparum
Author(s) -
Mery Budiarti,
Wahyu Jokopriyambodo
Publication year - 2020
Publication title -
buletin penelitian tanaman rempah dan obat/bulletin komunikasi penelitian tanaman rempah dan obat
Language(s) - English
Resource type - Journals
eISSN - 2527-4414
pISSN - 0215-0824
DOI - 10.21082/bullittro.v31n2.2020.85-96
Subject(s) - phytochemical , plasmodium falciparum , terpenoid , potency , maceration (sewage) , alkaloid , traditional medicine , chemistry , ethyl acetate , in vitro , stereochemistry , chromatography , biology , biochemistry , medicine , malaria , immunology , materials science , composite material
Paliasa ( Kleinhovia hospita ) is known as a plant that has been used empirically for malaria treatment, especially in Eastern Indonesia. However, scientific publications regarding to the antiplasmodial activity of these natural resources are still limited. The aim of this study was to examine the potency of paliasa leaves as antiplasmodial against Plasmodium falciparum parasite. The procedure included sample and extract preparation, antiplasmodial in vitro activity testing on P. falciparum strain 3D7, and phytochemical screening using Thin Layer Chromatography (TLC). The extracts and fractions were prepared through maceration process for 72 hours with 96% ethanol, then continued with multilevel liquid-liquid partition using hexane, ethyl acetate and 96% methanol. Antiplasmodial in vitro testing showed that ethyl acetate (IC 50 1.08 µg.ml -1 ) and hexane (IC 50 1.24 µg.ml -1 ) fractions were include to the highly activity category. The research samples contained alkaloids, triterpenoids and steroids as the major compounds. The terpenoid alkaloid compound was a cycloartane triterpenoid alkaloid that had been isolated from paliasa leaves. Therefore, it is assumed that the leaf of palasia has a compound with antiplasmodial activity. However, more research needs to be done to determine the active compound and the antiplasmodial mechanisms involved