Open AccessIsolation and characterisation of Vibrio alginolyticus lytic bacteriophage φVa-1 from brackishwater clam Meritrix meritrix in India
Author(s) -
E. C. Abhilash,
S.V. Alavandi
Publication year - 2017
Publication title -
indian journal of fisheries
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.216
H-Index - 12
ISSN - 0970-6011
DOI - 10.21077/ijf.2017.64.3.67421-17
Subject(s) - vibrio alginolyticus , lytic cycle , bacteriophage , microbiology and biotechnology , biology , lysin , myoviridae , vibrio , phage therapy , isolation (microbiology) , agar , bacteria , virology , virus , biochemistry , escherichia coli , genetics , gene
The Gram negative bacterium Vibrio alginolyticus is generally found in marine and brackishwater systems. A lytic bacteriophage capable of specifically infecting V. alginolyticus was isolated from the brackishwater clam (Meretrix meretrix) using agar overlay technique. The phage produced plaques 3 mm in dia, which increased to 5 mm overnight on tryptone soya agar (TSA) plates and the optimum temperature and pH was found to be 32°C and 7.5 respectively. The phage was designated as φVa-1 and nucleic acid characterisation confirmed that the phage has double stranded DNA. Transmission electron microscopic observations revealed that the bacteriophage had hexagonal structure with a long contractile tail andthe phage was found to belong to the family Myoviridae.