Open AccessEvaluation of Different Techniques in Recovering of Oocytes and Storage Duration of Ovaries on the Quality and Quantity of Bovine in Vitro Maturation
Author(s) -
Zaituni Udin,
Masrizal Masrizal,
Hendri Hendri,
Syafri Nanda
Publication year - 2020
Publication title -
buletin fakultas peternakan universitas gadjah mada/buletin peternakan
Language(s) - English
Resource type - Journals
eISSN - 2407-876X
pISSN - 0126-4400
DOI - 10.21059/buletinpeternak.v44i2.52181
Subject(s) - andrology , slicing , oocyte , ovary , biology , embryo , medicine , endocrinology , microbiology and biotechnology , computer science , world wide web
This study aimed to evaluate different recovery techniques of oocytes and the duration time between ovary collection and processing in the laboratory on the quality and quantity of Simmental cross bovine oocytes in vitro maturation. A total of 75 bovine ovaries were divided into three groups. Experiment-1; the storage duration of ovaries were 6, 9 and 12 h. Experiment -2; the techniques for oocytes recovery were slicing, aspiration and slicing + aspiration. The ovaries were collected from slaughterhouse and kept in saline solution during transportation to laboratory. A total of 136 oocytes were used for experiment-1, and 246 oocytes for experiment-2. This study found that the number and quality of oocytes were significantly different (P 0.05) on oocytes quality. The average of oocytes of groups slicing, aspiration and slicing plus aspiration were 14.66±2.09; 9.46±2.99 and 16.40±6.86, respectively. Both experiment was significant effect (P<0.05) on immaturation rate of bovine. Conclusion of this study the storage time of ovaries was suitable in 9 h and the technique of oocytes collection was in slicing plus aspiration.