Open AccessPreparation of Class Y Immunoglobulins that Neutralize the Marburg Virus
Author(s) -
О. А. Полежаева,
Anastasiya V. Zybkina,
А. В. Зайковская,
Oleg V. Pyankov,
С. А. Пьянков,
А. В. Семенова,
Г. В. Кочнева,
Д. Н. Щербаков
Publication year - 2021
Publication title -
problemy osobo opasnyh infekcij
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.16
H-Index - 3
eISSN - 2658-719X
pISSN - 0370-1069
DOI - 10.21055/0370-1069-2020-4-86-91
Subject(s) - virology , recombinant dna , vesicular stomatitis virus , virus , antibody , marburg virus , vesicular stomatitis , vaccinia , mononegavirales , titer , biology , recombinant virus , neutralizing antibody , paramyxoviridae , immunology , viral disease , ebola virus , gene , biochemistry
The aim was to study the possibility of inducing Marburg-neutralizing chicken antibodies (MARV) using various immunogens. Materials and methods . Recombinant vaccinia virus expressing the surface glycoprotein (GP) transgene MARV of Musoke strain and pseudovirus particles exhibiting GP of three strains of MARV – Popp, Musoke and DRC2000 based on lentivirus and recombinant strain of vesicular stomatitis virus (VSV) were used as immunogens. Two groups of birds were involved in the study. Chickens were immunized 9 times: first time they were injected with the recombinant vaccinia virus, and then 8 times – with pseudovirus particles (based on lentivirus and a recombinant strain of the vesicular stomatitis virus). The accumulation of specific antibodies was evaluated by enzyme-linked immunosorbent assay (ELISA). We used recombinant VSV exhibiting GP MARV, and natural MARV strain Popp for the analysis of accumulation of neutralizing antibodies. Results and discussion . We have developed an effective immunization schedule for chickens with three recombinant constructs presenting GP MARV, which results in the induction of chicken IgY antibodies against Marburg virus with a titer in ELISA from 1:100 to 1:1 million. The obtained IgY neutralize MARV pseudoviruses (Popp, DRC2000, Musoke) at a dilution of 1/256 to 1/1024 and the natural MARV virus of the Popp strain at a dilution of 1/8. More stable results were demonstrated by immunization using Freund’s incomplete adjuvant.