Open AccessMolecular Diagnostics of Coronavirus Infection in the Kyrgyz Republic
Author(s) -
С. В. Хегай,
А. К. Джапарова,
Э. М. Дуйшеналиева,
Bolot Kalmyrzaev,
К. Т. Касымбекова,
Tatyana Enverovna Kuchuk,
Nurbolot Usenbaev,
А. Д. Жунушов
Publication year - 2020
Publication title -
problemy osobo opasnyh infekcij
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.16
H-Index - 3
eISSN - 2658-719X
pISSN - 0370-1069
DOI - 10.21055/0370-1069-2020-2-141-143
Subject(s) - pooling , covid-19 , coronavirus , virology , rna , vector (molecular biology) , biology , computational biology , medicine , computer science , pathology , artificial intelligence , genetics , gene , disease , outbreak , infectious disease (medical specialty) , recombinant dna
. Objective of the study was to develop an effective method of sample pooling for the detection of SARSCoV-2 coronavirus RNA using PCR and evaluate that approach with various test systems. Materials and methods. SARS-CoV-2 coronavirus RNA was detected in samples containing nasal swabs placed in a transport medium. 5 samples were combined into one pool to perform the analysis. The effectiveness of the “in single test tube” pooling method for performing mass studies for COVID-19 was evaluated using the Vector-PCRrv-2019-nCoV-RG-19 test systems,Russia; “ArtTest COVID-19”,Belarus; “BioSpeedy”,Turkey. Results and discussion. A total of 587 pools were studied, consisting of 2935 test samples, in which 56 samples containing SARS-CoV-2 RNA were detected and confirmed by PCR. When studying the method of pooling samples, its specificity and optimal sensitivity for detecting SARS-CoV-2 RNA using the Vector-PCRrv-2019-nCoV-RG, ArtTest COVID-19, and BioSpeedy test systems were shown. The results of applying the pooling method correlated with the data obtained without pooling samples. The average deviation of the cycle amounted to 2 Ct; the fluorescence curve of positive samples corresponded to the «S» form.