Open AccessApplication of Dot-Immunoassay for Detection of Plague Agent Antigens in the Field Samples
Author(s) -
О. А. Носкова,
Т. Ю. Загоскина,
Е. Н. Субычева,
Е Ю Марков,
Ю. О. Попова,
Л. Г. Гриднева,
Е. П. Михайлов
Publication year - 2014
Publication title -
problemy osobo opasnyh infekcij
Language(s) - English
Resource type - Journals
eISSN - 2658-719X
pISSN - 0370-1069
DOI - 10.21055/0370-1069-2014-3-69-71
Subject(s) - immunoassay , plague (disease) , antigen , serology , heterologous , virology , chromatography , hemagglutination , biology , yersinia pestis , microbiology and biotechnology , chemistry , medicine , immunology , antibody , biochemistry , pathology , virulence , gene
CFU/ml) and soluble antigens (FI) − ≥ 4.8 ng/ml and high specificity, confirmed by the absence of false-positive reactions with five heterologous microorganisms. The test-systems were used for Y. pestis antigen detection in field material from the territory of the Altai mountain natural plague focus by dot-immunoassay with comparison of the received results in passive hemagglutination reaction. Test-systems possessed a number of advantages as compared to routine serological reactions and could be applied with success by practical public health services both in stationary and field conditions.