Open AccessApplication of Freeze-Dryers of Chamber Type in the Collections of Pathogenic Microorganisms
Author(s) -
Н. С. Червякова,
Т. В. Валова,
А. В. Осин
Publication year - 2014
Publication title -
problemy osobo opasnyh infekcij
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.16
H-Index - 3
eISSN - 2658-719X
pISSN - 0370-1069
DOI - 10.21055/0370-1069-2014-3-65-68
Subject(s) - microorganism , bacteria , pathogenicity , microbiology and biotechnology , pathogenic bacteria , freeze drying , biology , chemistry , chromatography , genetics
By the example of Martin Christ Epsilon 2-6D device carried out was assessment of the possibility to use freeze-dryers of the chamber type for conservation of pathogenic microorganisms collection strains. Elaborated was algorithm of lyophilisation of the III-IV pathogenicity groups bacteria, which incorporated conditions of freeze-drying and biological safety provision of this process. Indices of viability and survivability were defined for freeze-dried cells of pathogenic bacteria strains. Using thermostability test calculated were predicted timelines of storage of collection strains preparations freeze-dried in the flasks in Martin Christ Epsilon 2-6D. It was determined that in the collections of pathogenic microorganisms freeze-dryers of the chamber type could be used most prospectively for the III-IV pathogenicity groups bacteria conservation requiring mass reproduction and not intended for long storage. At the same time their application for freeze-drying of the strains of the I-II pathogenicity groups bacteria intended for a long storage, requires further adaptation of these devices as regards biological safety provision and prolongation of the shelf life.