Open AccessDevelopment of a Set of Primers for Drug-Resistance Genes Detection in the Agents of Dangerous Bacterial Infections as Exemplified by <I>Yersinia pestis</I>, <I>Vibrio cholerae</I>, <I>Escherichia coli</I> Strains
Author(s) -
К. А. Никифоров,
Л. В. Анисимова,
Г. Н. Одиноков,
А. В. Фадеева,
Л. А. Новичкова,
Г. А. Ерошенко,
Владимир Кутырев
Publication year - 2014
Publication title -
problemy osobo opasnyh infekcij
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.16
H-Index - 3
eISSN - 2658-719X
pISSN - 0370-1069
DOI - 10.21055/0370-1069-2014-3-57-60
Subject(s) - yersinia pestis , microbiology and biotechnology , kanamycin , pathogenic bacteria , biology , tetr , vibrio cholerae , plasmid , polymyxin , escherichia coli , streptomycin , virology , drug resistance , bacteria , gene , antibiotics , genetics , virulence , repressor , transcription factor
A set of primers for detection of genes encoding resistance to streptomycin ( strA, strB ), tetracyclin ( tetA, tetR ), chloramphenicol ( catА ), kanamycin ( npt , aphA ), vankomycin ( sanA ), polymyxin ( pmrD ) has been developed with the aim of rapid and effective detection of drug-resistant strains of dangerous bacterial infections agents. Efficacy of constructed primers has been confirmed against a panel of 40 Yersinia pestis, 49 Vibrio cholerae, and 2 Escherichia coli strains from the State collection of pathogenic bacteria of the RAPI “Microbe”. Drug-resistance genes ntp and catA have been detected in plague agent strains , strA, strB , npt , aphA , tetA and tetR - in cholera agent; strA , tetR , ntp and aphA - in pathogenic strain E. coli О157:H7. Determined is universal character of the designed primers for drug-resistance genes detection in these pathogenic bacteria species.