Open AccessProduction of Rabbit Anti-Rabies Immunoglobulin Using Cultural Antigen
Author(s) -
С. В. Генералов,
Е. Г. Абрамова,
Ж. В. Матвеева,
И. М. Жулидов,
О. А. Лобовикова,
L. V. Savitskaya,
Л. Н. Минаева,
М. В. Галкина,
Tatiana Mikheeva,
А. В. Комиссаров,
М. Н. Киреев,
А. К. Никифоров
Publication year - 2012
Publication title -
problemy osobo opasnyh infekcij
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.16
H-Index - 3
eISSN - 2658-719X
pISSN - 0370-1069
DOI - 10.21055/0370-1069-2012-2(112)-78-81
Subject(s) - rabies virus , rabies , antibody , heterologous , virology , antigen , titer , immunoassay , lyssavirus , rhabdoviridae , immunization , biology , chemistry , virus , immunology , biochemistry , gene
Justified is the possibility of application of fixed rabies virus Moscow 3253, reproduced on the cell culture Vero, as an antigen for heterologous anti-rabies immunoglobulin production. Application of the adjuvant - aluminium hydrate - is determined to be effective. Herein, by the day 73 post immunization, specific antibodies titer is ≥ 1:500 (wherein specific activity has been identified by means of neutralization test, carried out on white mice, and dot-blot immunoassay). The level of specific activity in experimental samples of anti-rabies immunoglobulin, isolated from rabbit immune serum, corresponds to 332 and 347 ME/ml. Physicochemical and biological properties of anti-rabies immunoglobulin, produced with the help of cultural antigen, fully comply with regulatory requirements specified for commercial preparation of heterologous anti-rabies immunoglobulin.