Open AccessMolecular Genetic Analysis of the Complete Genome of Tick-Borne Encephalitis Virus (Siberia Subtype): Modern Kolarovo-2008 Isolate
Author(s) -
Е. В. Чаусов,
V. A. Ternovoy,
Е. В. Протопопова,
Svetla. Konovalova,
Yu. V. Коova,
Н. Л. Тупота,
Н. С. Москвитина,
В. Н. Романенко,
Н. В. Иванова,
Н. П. Большакова,
Г Н Леонова,
В Б Локтев
Publication year - 2011
Publication title -
problemy osobo opasnyh infekcij
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.16
H-Index - 3
eISSN - 2658-719X
pISSN - 0370-1069
DOI - 10.21055/0370-1069-2011-4(110)-44-48
Subject(s) - biology , genome , virology , homology (biology) , tick borne encephalitis virus , encephalitis , virus , tick borne encephalitis , genetics , gene , nucleic acid sequence , whole genome sequencing , tick , sequence analysis , encephalitis viruses
Determined is the complete genome sequence of Kolarovo-2008 strain (Siberia subtype) of Tick-borne encephalitis virus (TBEV), isolated from a tick in the suburbs of the Tomsk city. Nucleotide sequence analysis testifies of the fact that the level of genetic differences within the Siberian subtype of TBEV amounts to 10 % of the nucleotide sequence and to 7 % of amino-acid sequence for certain virus genes. 3'-HTO of the genome of Siberian subtype has the highest rate of variability and the homology level ranging from 65 to 97 %. Kolarovo-2008 and Vasilchenko (isolated in Novosibirsk in 1969) strains have the highest level of genome homology. The level of dissimilarity between the two Tomsk strains is substantially higher: the total number of amino-acid substitutions in Tomsk Zausaev and Kolarovo-2008 strains equals to 124, and 3'HTO level of homology is 79 %. Identified genetic variability of the Siberian subtype of TBEV is of a great importance for further development and enhancement of tick-borne encephalitis virus diagnostics.