Open AccessDevelopment of the Multiplex Real-Time PCR for Marburg, Ebola, and Lassa Viruses Identification
Author(s) -
А. О. Семенцова,
А. Н. Шиков,
V. A. Ternovoy,
Анна Викторовна Винокурова,
Н. Е. Костина,
E. A. Stavsky,
А. П. Агафонов
Publication year - 2011
Publication title -
problemy osobo opasnyh infekcij
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.16
H-Index - 3
eISSN - 2658-719X
pISSN - 0370-1069
DOI - 10.21055/0370-1069-2011-3(109)-64-67
Subject(s) - virology , multiplex , marburg virus , lassa fever , real time polymerase chain reaction , identification (biology) , ebola virus , lassa virus , biology , outbreak , virus , bioinformatics , gene , genetics , botany
Presented are the data on the development and approbation of the method of Marburg, Ebola, and Lassa viruses identification based on real-time multiplex PCR with hybridization-fluorescent detection. This method is meant for the differential diagnostics of hemorrhagic fevers caused by these viruses. Displayed are the results of determination of multiplex PCR analytical sensitivity and specific activity.