Open AccessOptimization of Reproduction Stage in Technology of Production of Plague Diagnostic Bacteriophage L-413C
Author(s) -
Т. В. Аленкина,
О. С. Зинина,
М. В. Антонычева,
Н. И. Вахрушина,
А. К. Никифоров
Publication year - 2011
Publication title -
problemy osobo opasnyh infekcij
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.16
H-Index - 3
eISSN - 2658-719X
pISSN - 0370-1069
DOI - 10.21055/0370-1069-2011-2(108)-79-82
Subject(s) - plague (disease) , bacteriophage , yeast , reproduction , biology , nutrient , food science , microbiology and biotechnology , biochemistry , escherichia coli , genetics , medicine , ecology , pathology , gene
New nutrient media based on baker yeast autolizate were used for the first time for manufacturing of diagnostic preparation of plague bacteriophage L-413C. Experimental media provide high concentration of phage particles at the stage of propagation, and good survivability in lyophilization. Media in which yeast autolizate was a nutrient protein basis appeared to be more effective than those in which it was a stimulating additive. Phage preparations preserved stability of properties during storage at 4-8 °С, and at a higher temperature in the test of accelerated aging. Introduction of yeast nutrient media in technology of plague diagnostic bacteriophage L-413C manufacturing opens good prospects for increasing of production efficiency and decreasing of cost value of the preparation