
Identification and Intraspecific Typing of Plague Microbe Strains with Their Potential Virulence Determination Using PCR
Author(s) -
А. В. Гаева,
E. G. Boolgakova,
I. Yu. Sukhonosov,
Л. В. Анисимова,
Л. А. Новичкова,
Владимир Кутырев
Publication year - 2011
Publication title -
problemy osobo opasnyh infekcij
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.16
H-Index - 3
eISSN - 2658-719X
pISSN - 0370-1069
DOI - 10.21055/0370-1069-2011-2(108)-36-41
Subject(s) - virulence , intraspecific competition , typing , biology , multiplex polymerase chain reaction , polymerase chain reaction , plague (disease) , plasmid , microbiology and biotechnology , identification (biology) , multiplex , genetics , gene , zoology , geography , botany , archaeology
Developed is the method of identification and intraspecific typing of plague microbe strains along with their potential virulence determination. Intraspecific differentiation and focal attribution of the examined plague microbe strains can be determined by monolocus VNTR-PCR, and main virulence determinants (chromosomal pigmentation region and calcium-dependence plasmid genes) - by multiplex PCR.