Open AccessImmune-Magnetic Absorbents Used for Express Diagnosis of Dangerous Infectious Diseases: Biotechnology Aspects and Experience of Application
Author(s) -
I. S. Tyumentseva,
Е. Н. Афанасьев,
L. V. Lyapustina,
О. И. Коготкова,
И. В. Жарникова,
V. Efremenko,
Д. А. Будыка,
Н. Ф. Василенко,
E. E. Afanas`eva,
А. Н. Куличенко
Publication year - 2009
Publication title -
problemy osobo opasnyh infekcij
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.16
H-Index - 3
eISSN - 2658-719X
pISSN - 0370-1069
DOI - 10.21055/0370-1069-2009-3(101)-59-61
Subject(s) - adsorption , immune system , pathogen , reliability (semiconductor) , facilitation , chemistry , materials science , nanotechnology , chemical engineering , microbiology and biotechnology , biology , immunology , engineering , organic chemistry , neuroscience , physics , power (physics) , quantum mechanics
Compositional micro-grained immune-magnetic absorbents (IMA) were obtained on the basis of silica - aluminocilicate, modified by polyglucin and sodium secondary alkylsulphate. IMA were shown to possess high adsorption activity due to their developed surface and implanted specific ligand, standard structural features, mechanical reliability, chemical and microbiological stability. The existence of magnetic material provided facilitation and convenience of operations with absorbents in analysis performance. Applying of IMA allowed to release of any mixtures with their adverse effect on reaction during samples preparing by means of rinsing the absorbent with fixed infected agent on it; concentrating the unknown pathogen to the extent possible increased the specificity and sensitivity of express-analysis methods (EIA and PCR), the time spending for analysis reduced substantially (up to 1-3hours).