Open AccessProduction of Monoclonal Antibodies against Outer Membrane Proteins of <i>Burkholderia pseudomallei</i>, Strain C-141
Author(s) -
С. С. Ветчинин,
P. C. Kopylov,
Н. В. Киселева,
А. М. Баранов,
Е. В. Баранова,
Elena V. Galkina,
Alexander I. Borzilov,
I. Y. Kalachev
Publication year - 2008
Publication title -
problemy osobo opasnyh infekcij
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.16
H-Index - 3
eISSN - 2658-719X
pISSN - 0370-1069
DOI - 10.21055/0370-1069-2008-4(98)-54-57
Subject(s) - burkholderia pseudomallei , monoclonal antibody , clone (java method) , burkholderia , microbiology and biotechnology , antibody , bacterial outer membrane , biology , strain (injury) , antigen , chemistry , bacteria , escherichia coli , gene , biochemistry , immunology , genetics , anatomy
Monoclonal antibodies (MAb) were produced against two B. pseudomallei high-purified membrane proteins with Mr 29 kDa (p29) and 45 kDa (p45). Monoclonal antibodies from culture supernatant fluids of 4F2 and 1G11 clones showed specific interaction with protein moiety of p29 both Burkholderia pseudomallei and Burkholderia mallei in ELISA and Western blotting. However, MAb of 3G4 clone were bound to the LPS-protein structures of these microbial cells. Analysis of interaction of Mabs from 4F2 and 1G11 clones with antigens of different lysates of pathogenic cells confirmed high specificity of these antibodies to p29 membrane protein of B. pseudomallei and B. mallei .