Open AccessStructural and Functional Analysis of <i>nap</i> Operon Genes in <i>Yersinia pestis</i> Strains of Different Subspecies
Author(s) -
Г. Н. Одиноков,
Г. А. Ерошенко,
N. A. Vidyaeva,
Ja. M. Krasnov,
N. P. Gouseva,
Владимир Кутырев
Publication year - 2008
Publication title -
problemy osobo opasnyh infekcij
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.16
H-Index - 3
eISSN - 2658-719X
pISSN - 0370-1069
DOI - 10.21055/0370-1069-2008-4(98)-40-42
Subject(s) - subspecies , napa , operon , biology , yersinia pestis , genetics , gene , denitrifying bacteria , microbiology and biotechnology , chemistry , escherichia coli , virulence , zoology , organic chemistry , nitrogen , denitrification
Carried out was structural and functional analysis of nap genes coding for a significant diagnostic feature - nitrate reduction in main and non-main subspecies of Yersinia pestis . The presence of a single nucleotide substitution (A for T in position 631) in gene napA was determined to be the reason for the lack of nitrate reduction in part of the main subspecies strains. Other mutation - single nucleotide substitution G for A in position 1021 of napA is not the reason for absence of this diagnostic feature in non-main subspecies and biovar microtus as this substitution is present in denitrifying and nondenitrifying strains.